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Isotype control pair in red. WT: wild type, KO: knockout. This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption: Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. These three subpopulations were purified by cell sorting on a FACS Vantage, and their morphology was evaluated ex vivo from cytospin slides F , or after in vitro culture in chamber slides for 12 h G.
Image caption: Zymosan and T. Sequential gates were made as shown in Fig. Plots show the frequencies of each subpopulation. Data are representative of more than 3 independent experiments. Titles above plots indicate in vivo – in vitro stimulations.
Values inside gates represent the frequencies of ILproducing cells in each subpopulation. Nuclei were counterstained with Hoechst Image caption: Heterogeneous phenotype of thymic resident myeloid cells.
Red circles mark the three different cell populations discussed in the text. In this experiment, populations 1, 2 and 3 represented 0. While the absolute cell number varied as a result of the preparation, the percentage of each cell type relative to each other was consistent over many experiments. Populations were identified as in A and stained with the antibodies as indicated. Isotype controls red lines were used to correct differences in the autofluorescence of each cell population.
Data are representative of more than ten experiments a and b and three experiments c. Thymuses from wild type B6 mice were harvested and frozen post-irradiation at the indicated times 1 hour through 24 hours. Panel labeled \”control\” was a section stained with secondary antibody only. Original magnification was X. Image caption: Influx of innate cells rapidly declines twenty-four hours after irradiation.
Thymuses were harvested from mice at the indicated times after irradiation. Numbers in the plots are the percentage of cells within the electronic gate. The mean is shown by a horizontal bar and statistics were calculated with 2-tailed, nonpaired Mann-Whitney test. Percent of cells in each area is indicated in the Figure.
Individual stains are in Supplementary Figure 3. The tumors were immunostained with a CDAlexa Flour antibody to recognize endothelial cells that comprise blood vessels. Image caption: Histological and immunohistochemical characterization of cell infiltrates during ALA. B PAS staining shows E. PLoS Pathog 9 1 : e From: Laporte et al. Journal of Neuroinflammation C Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF treated mice at 1 h, 1 day, and 4 days post-injury.
D Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining.
Sections were stained with DAPI blue to show all nuclei. Ipsi: ipsilateral cortex; Contra: contralateral cortex. Magnification x Representative photomicrographs of sections stained for T-cells anti-CD3 are shown A. Macrophage surface was quantified in the mucosa and the number of macrophages was counted in the muscle. The numbers of Ki67 positive cells were counted per crypt and representative photomicrographs are shown D. A and C Original magnification x, D x Each symbol represents 1 animal.
Scale bars represent 1mm. Image caption: Clodronate liposome mediated macrophage depletion in Col4a3KO mice. Animals were entered in the study at the age of 4 weeks and were continually dosed until the age of 8 weeks. Injections were repeated every second day, except for the first two doses, which were injected on consecutive days.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. Original magnification x C Representative micrographs of Ly-6B.
D Quantitative analysis of Ly-6B. Immunofluorescent detection for CXCL1 red and liver cells nuclei blue for nuclei first shows CXCL1 expression in the sinusoids throughout liver parenchyma.
C Higher resolution shows that CXCL1 red is expressed by sub-endothelial cells, which also store retinol droplets in separate compartments, as shown by CRBP1 staining green. TOPRO3 was used for nuclei vizualisation. Figure A. Figure B. Neuroprotective pentapeptide CN improves functional and histological outcomes in a murine model of intracerebral hemorrhage. Sci Rep. Image caption: Renal markers of inflammation at Week Image caption: Focal expression of Irga6 in healthy mouse liver and kidney.
For Irga6 and CD3 double staining in liver, consecutive serial sections were analysed numbered 1, 2, 3, 4. Spontaneous focal activation of invariant natural killer T iNKT cells in mouse liver and kidney. BMC Biol. Published customer image: Rat anti Mouse F, clone A MCAR used for the demonstration of Kupffer cells in mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Image caption: Combination of bone fracture and alendronate treatment causes loss of myogenic cells.
PBS Control group or alendronate Alendronate group was subcutaneously injected three times at seven-day intervals. The femur was broken and vastus lateralis muscle injured by cold shock on day Then the vastus lateralis muscle was removed on day 1, 4, or 7 after the bone fracture and cold injury.
Arrows represent M-cadherin-positive muscle satellite cells in C. Images represent a part of each original picture. Numbers of CDpositive capillaries per mm 2 of each original picture are shown in O and R.
From: Kawada S, Harada A, Hashimoto N Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment. PLoS Genet 13 8 : e The images are representative of at least 5 mice in each strain. A 1 day; B 2 days. For all panels, representative images are shown and arrows denote positive staining. Embelin can protect mice from thioacetamide-induced acute liver injury.
Biomed Pharmacother. Interstitial inflammation is a hallmark feature of kidney fibrosis. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy. Physiol Rep. All experiments performed on J Data acquired on the ZE5 Cell Analyzer. Numbers indicate the percentage of cells within the circles. Apoptotic cells were detected by TUNEL green and their cortical localization was visualized by counterstaining of thymic sections with an antibody against DEC red.
Sections are representative of multiple samples from more than ten experiments. Image caption: L-tryptophan administration reverses stress-induced alterations on inflammatory response and lipid peroxidation.